是一種氨基糖苷類抗生素，是穩(wěn)定轉(zhuǎn)染最常用的抗性篩選試劑。通過抑制轉(zhuǎn)座子Tn601，Tn5的基因，干擾核糖體功能而阻斷蛋白質(zhì)合成，對(duì)原核和真核等細(xì)胞產(chǎn)生毒素。當(dāng)neo基因被整合進(jìn)真核細(xì)胞DNA后，則能啟動(dòng)neo基因編碼的序列轉(zhuǎn)錄為mRNA，從而獲得抗性產(chǎn)物氨基糖苷磷酸轉(zhuǎn)移酶的高效表達(dá)，使細(xì)胞獲得抗性而能在含有G418的選擇性培養(yǎng)基中生長(zhǎng)。

使用本產(chǎn)品的應(yīng)用案例（僅供參考）

In Vitro

Cell（HepG2.2.15 cells，380 μg/mL G-418 sulfate）

Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Solarbio) and supplemented with 10% fetal bovine serum (FBS).G-418 sulfate (380 μg/mL; Solarbio) was added to DMEM with 10% FBS to maintain HepG2.2.15 cells. All cells were incubated at 37°C with 5% CO2.

來源文獻(xiàn)：Guo C, Ouyang Y, Cai J, Xiong L, Chen Y, Zeng X, Liu A. High expression of IL-4R enhances proliferation and invasion of hepatocellular carcinoma cells. Int J Biol Markers. 2017 Oct 31;32(4):e384-e390. doi: 10.5301/ijbm.5000280. PMID: 28665449.

Cell（（HepG2.2.15 cells，300 μg/mL G-418 ）

HepG2.2.15 cells were incubated in minimum Eagle’s medium containing 10% FBS and 300 μg/mL G418 (Solarbio, Beijing, China). 

來源文獻(xiàn)：Jiang W, Wang L, Zhang Y, Li H. Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/TNFAIP3 Axis. Cancer Manag Res. 2020 Nov 2;12:11031-11040. doi: 10.2147/CMAR.S272983. PMID: 33173336; PMCID: PMC7648158.

Cell（OC cells，800 μg/mL  G418）

PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Solarbio, Shanghai, China) at a concentration  of 800 μg/mL and puromycin (Solarbio, Shanghai, China) at a concentration of 2 μg/mL. 

來源文獻(xiàn)：Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.

Cell（Replicon cell ，500 μg/ml G418）

Replicon cell lines were selected and maintained in 500 μg/ml G418 (Geneticin; Solarbio, China). 

來源文獻(xiàn)：Sobhanimonfared F, Bamdad T, Roohvand F. Cross talk between alcohol-induced oxidative stress and HCV replication. Arch Microbiol. 2020 Sep;202(7):1889-1898. doi: 10.1007/s00203-020-01909-9. Epub 2020 May 24. PMID: 32448963.