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試劑
我們的試劑品牌很多，都是高品質的試劑
無菌甘油
基本售價：130.00元
【保存條件】室溫保存，一年有效【概述】甘油又名丙三醇，是一種無色、無臭、味甘的粘稠液體。 分子式：C3H8O3，分子量：92.094，CAS號：56-81-5。本產品純度高于99%，經無菌處理，使用時應稀釋至工作濃度。【注意事項】1. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。2. 本品為有毒化合物，避免與皮膚和眼睛接觸，請小心操作。3. 溶液為無菌溶液，使用時應盡量避免污染。
鮭魚精DNA 10mg/ml
基本售價：100.00元
鮭魚精DNA 10mg/ml產品簡介別名單鏈DNA儲存條件-20℃，有效期1年規格1ml單位支Solarbio生產的鮭魚精DNA溶液（10mg/mL）是經過酚氯仿抽提，超聲和熱變性處理的短片段的單鏈DNA溶液，可直接用于Southern、Northern等核酸雜交中。 本產品鮭魚精DNA的濃度為10mg/mL，使用時按實驗具體要求操作，稀釋至所需工作濃度即可。 鮭魚精DNA注意事項： 1. 如果每次的使用量很小，可以適當分裝后再使用，避免反復凍融。 2. 為了您的安全和健康，請穿實驗服并戴手套操作。
伊文斯藍染色液(0.5%)
基本售價：150.00元
伊文斯藍染色液(0.5%)產品簡介CAS314-13-6英文名稱Evan's Blue Stain Solution, 0.5%別名；伊文思蘭；偶氮藍；依文氏蘭；依文思藍；依文斯蘭；埃文斯藍；6,6'-[[3,3'-二甲基(1,1'-二苯基)-4,4'-二基]雙(偶氮基)]雙(4-氨基-5-羥基-1,3-萘二磺酸)四鈉鹽分子式C34H24N6Na4O14S4分子量960.81儲存條件室溫,避光保存,有效期1年規格100mL 500mL單位瓶伊文斯蘭(Evans Blue)又稱偶氮藍。伊文斯蘭與臺盼藍都是細胞活性染料，常用于檢測細胞膜的完整性和細胞是否存活。活細胞因有外排功能而無法被伊文斯蘭染成藍色，而死細胞會被染成淡藍色。因此可以通過此方法在顯微鏡下區分死細胞與活細胞，但無法區分死亡與壞死。備注：以上數據均來自公開文獻, Solarbio暫未進行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
赤霉素(GA3)
基本售價：80.00元
赤霉素(GA3)產品簡介英文名稱Gibberellic acidCAS77-06-5級別BR別名赤霉酸；赤霉素A；2,4a,7-三羥基-1-甲基-8-亞甲基赤霉-3-烯-1,10-二羧酸-1,4a-內酯；九二零純度90.0%分子式C19H22O6分子量346.37外觀（性狀）白色粉末儲存條件RT有效期2年溶解性50mg/ml EtOH規格5g單位瓶赤霉素是植物生長激素，能促進莖、葉的生長，提早抽苔開花，促進種子、塊莖、塊根發芽，刺激果實生長,增加結果率或形成無籽果實等.結馬鈴薯、蕃茄、稻、麥、棉花、大豆、豌豆、煙草、果樹等均有增產作用。但施用過多的赤霉素，植物會出現黃而細長的枝條，即失綠、徒長現象，反而影響產量。赤霉素還可用于從大麥制造麥芽。它對昆蟲的發育也有促進作用。能溶于醇、酮、酯類等有機溶劑和pH值6.3的磷酸緩沖溶液，難溶于醚、氯仿、苯和水。與鉀、鈉離子形成鹽并溶于水。赤霉素固體比較穩定，在干燥、密閉條件下貯存時間較久不會失效；水溶液不穩定，低溫時短期貯存不致失效，溫度較高時分解較快；酸性或弱酸性條件較穩定，堿性條件不穩定。 備注：產品信息可能會有優化升級。請以實際標簽信息為準。 備注：以上數據均來自公開文獻, Solarbio暫未進行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
維生素C
基本售價：240.00元
維生素C產品簡介CAS50-81-7中文名稱維生素C英文名稱L-Ascorbic Acid別名L(+)-Ascorbic acid;Vitamin C;L-抗壞血酸純度HPLC≥98%分子式C6H8O6 分子量176.12外觀（性狀）White to off-white Solid儲存條件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥20mg/mLMDLMFCD00064328ECEINECS 200-066-2InChIKeyCIWBSHSKHKDKBQ-JLAZNSOCSA-NInChIInChI=1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1PubChem CID54670067SMILESOC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O描述L-Ascorbic Acid是一種有效的還原劑和抗氧化劑。(L-Ascorbic Acid is a potent reducing agent and antioxidant.)生物活性L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[1].In VitroThe anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[1].In VivoL-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[2].數據來源文獻[1]. Sungrae Cho, et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018 Jul 27;8(1):11372.[2]. Satyanarayana Sreemantula, et al. Influence of antioxidant (L- ascorbic acid) on tolbutamide induced hypoglycaemia/antihyperglycaemia in normal and diabetic rats. BMC Endocr Disord. 2005 Mar 3;5(1):2.規格20mg 10mM*1mL (in Water) 100mg單位瓶一種重要的營養素和氧化還原劑，在骨鹽代謝及骨形成中具有重要作用。在體外，它能增加鈣鹽的沉積，促進礦化結節的形成，并通過間充質細胞間接調節破骨細胞的分化。維生素C能直接抑制核因子κB受體活化子配體（RANKL）誘導的破骨細胞形成 。 使用本產品的應用案例（僅供參考） In VitroCell（Osteogenic/成骨誘導；50 mg/l ascorbic acid） The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio). 數據來源文獻：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821. Cell（Osteogenic/成骨誘導；50 mg/l ascorbic acid） Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining. 數據來源文獻：Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421. In Vivo Rat（雄性新生SD Rat；100 mg vitamin C/kg bw/d；灌胃；30d） Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90. 來源文獻：Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635. 備注：以上數據均來自公開文獻, Solarbio暫未進行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods
PerCP/Cyanine5.5 Anti-Human CD3抗體
基本售價：840.00元
PerCP/Cyanine5.5 Anti-Human CD3 Antibody[OKT-3]Uniprot : P07766 貨號: E-AB-F1001J 應用: FCM Isotype: Mouse IgG2a, κ 宿主: Mouse Antigen Details Alternate NamesT-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3ESwissprotP07766 References1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.3. Lanier L, et al. 1986. J. Immunol. 137:2501.Product Details FormLiquidClone No.OKT-3 (See Other Available Formats) HostMouseIsotypeMouse IgG2a, κIsotype ControlPerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J] ReactivityHumanApplicationFCM (See the Application Description) Storage BufferPhosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.Recommended UseEach lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.ShippingBiological ice pack at 4℃Stability & StorageKeep as concentrated solution.Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.Centrifuge before opening to ensure complete recovery of vial contents.This product is guaranteed up to one year from purchase.ConjugationPerCP/Cyanine5.5PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).如何通過光譜圖選擇合適的檢測通道？ Background CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
豬血清（無菌過濾）
基本售價：1100.00元
豬血清（無菌過濾）貨號：S9060 保存：-20°C 有效期：5 年 產品說明： 本產品采用豬血液為原料，無菌采集、批量混合，經 0.1μm 微孔濾芯無菌過濾分裝而成。 本品未經過滅活，溶液狀態下為黃色澄清液體，無溶血或異物，長時間靜置會有少量蛋白析出，使用前請震蕩混勻。 產品應用（僅供參考）： 本品適合用于普通細胞培養，也可用于配制試劑用。 使用時需要注意以下幾點： 1. 血清一般儲存于-20℃，使用時為避免反復凍融，建議無菌環境下分裝成小包裝，儲存于-20℃。 2. 血清融化時最好先置于 2-8℃冰箱中使之溶解，然后在室溫下使之全溶，溶解過程中必須規則地搖晃均勻。 3. 融化后的血清在 4℃不宜長時間存放，應盡快使用 注意事項： 1. 產品信息僅供參考，如有疑問請致電 400-968-6088 咨詢。 2. 本產品僅供科研使用。請勿用于醫藥、臨床診斷或治療，食品及化妝品等用途。請勿存放于普通住宅區。 3. 為了您的安全和健康，請穿好實驗服并佩戴一次性手套和口罩操作。
轉鐵蛋白
基本售價：170.00元
轉鐵蛋白產品簡介英文名稱 Transferrin CAS 11096-37-0 純度 min.98% 外觀（性狀） 白色至淺黃色固體 儲存條件 2-8℃ 有效期 2年 溶解性 10 mg/mL in water 規格 10mg 單位 支 產品說明： 轉鐵蛋白又名運鐵蛋白 transferrin，TRF，siderophilin）是血漿中主要的含鐵蛋白質，負責運載由消化管吸收的鐵和由紅細胞降解釋放的鐵。以 TRF-Fe3+的復合物形式進入骨髓中，供成熟紅細胞的生成。 轉鐵蛋白是一種能傳遞鐵離子的蛋白質。用于生化研究。 別名：鐵傳遞蛋白 鐵蛋白 人轉鐵蛋白 純度：98% 溶解性：10 mg/mL in water 注意事項： 1. 我司生產的生化試劑如無特殊標注，基本為非無菌包裝，若用于細胞實驗，請提前做好預處理。 2. 一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。 3. 產品信息僅供參考，如有疑問請致電 400-968-6088 咨詢。 4. 本產品僅供科研使用。請勿用于醫藥、臨床診斷或治療，食品及化妝品等用途。請勿存放于普通住宅區。 5. 為了您的安全和健康，請穿好實驗服并佩戴一次性手套和口罩操作。
總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）
基本售價：200.00元
總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）產品簡介儲存條件2-8℃有效期6個月中文名稱總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）英文名稱Total Antioxidant Capacity(T-AOC) Assay Kit（FRAP Method）檢測方法可見分光光度法可見分光光度法 Spectrophotometer單位盒規格50T/48S測定意義：測定對象中各種抗氧化物質和抗氧化酶等構成總抗氧化水平。在生物學、醫學和藥學研究中常常檢測血漿、血清、唾液、尿液等各種體液，細胞或組織等裂解液、植物或中草藥抽提液及各種抗氧化物(antioxidant)溶液的總抗氧化能力。 測定原理：在酸性環境下，物質還原Fe3+-三吡啶三吖嗪(Fe3+-TPTZ)產生藍色的Fe2+-TPTZ的能力反映了其總抗氧化能力。 自備實驗用品：可見分光光度計、恒溫水浴鍋、低溫離心機、1mL玻璃比色皿和蒸餾水。 相關論文：A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice - PubMed (nih.gov)
大鼠白介素11(IL-11)酶聯免疫吸附測定試劑盒
基本售價：3200.00元
Rat IL-11(Interleukin 11) ELISA Kit產品貨號:E-EL-R0562c 中文名:大鼠白介素11(IL-11)酶聯免疫吸附測定試劑盒別稱:IL11, AGIF, IL-11, interleukin 11用途 該試劑盒用于體外定量檢測Rat 血清，血漿或其他生物體液中IL-11濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗大鼠IL-11抗體包被于酶標板上，實驗時樣品或標準品中的大鼠IL-11會與包被抗體結合，游離的成分被洗去。依次加入生物素化的抗大鼠IL-11抗體和辣根過氧化物酶標記的親和素。抗大鼠IL-11抗體與結合在包被抗體上的大鼠IL-11結合、生物素與親和素特異性結合而形成免疫復合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現藍色，加終止液后變成黃色。用酶標儀在450nm波長處測OD值，IL-11濃度與OD450值之間呈正比，通過繪制標準曲線計算出樣品中IL-11的濃度。反應類型 夾心法規格 96T反應時間 3.5h反應性 Rat檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的大鼠IL-11，且與其它相關蛋白無明顯交叉反應。重復性 板內，板間變異系數均<10%。典型數據 由于實驗操作條件的不同（如操作者、移液技術、洗板技術和穩定條件等），標準曲線的OD值會有所差異。以下數據和曲線僅供參考，實驗者需根據自己的實驗建立標準曲線。(pg/mL) O.D Average Corrected 10002.4832.5052.4942.4195001.6431.6991.6711.5962500.990.9660.9780.9031250.450.4760.4630.38862.50.2740.2520.2630.18831.250.1820.170.1760.10115.630.1180.1360.1270.05200.0730.0770.075--精密度板內精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.98127.48499.3948.54135.62511.36Standarddeviation 3.056.9720.282.997.5119.12C V (%) 6.365.474.066.165.543.74回收率 分別往5個不同樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 94-109101EDTA plasma (n=8) 96-112102Cell culture media (n=8) 90-10195線性 分別往5個樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)93-10795-10996-110Average (%)981021031:4Range (%)91-10979-9285-97Average (%)9985901:8Range (%)89-10280-9387-100Average (%)9686921:16Range (%)93-10679-9387-99Average (%)1008694試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規格 保存條件 ELISA酶標板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標準品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標準品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產品說明書Product Description1 份質檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發和微生物的污染。試劑體積以實際發貨版說明書為準。相關試劑在分裝時會比標簽上標明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應板孔中加入100μL標準品工作液或樣本，37℃孵育90分鐘2. 棄掉板內液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內液體，洗板3次4. 每孔加入100μL HRP酶結合物工作液，37℃孵育30分鐘，棄掉板內液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數，處理數據
Recombinant human IL-6 protein, C-His
基本售價：3980.00元
Recombinant human IL-6 protein, C-His產品編號bs-41177P英文名稱Recombinant human IL-6 protein, C-His中文名稱重組人白介素6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理論分子量15kDa檢測分子量21 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Human序 列30-212/212純 度>95% as determined by SDS-PAGE純化方法AC內毒素Not analyzed表達系統E.coli活性Not tested標簽C-His緩 沖 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產品介紹Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569產品圖片
Recombinant mouse IL-6 protein, C-His
基本售價：1280.00元
Recombinant mouse IL-6 protein, C-His產品編號bs-10861P英文名稱Recombinant mouse IL-6 protein, C-His中文名稱重組小鼠白介素IL-6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理論分子量23kDa檢測分子量26 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Mouse序 列25-211/211純 度>95% as determined by SDS-PAGE純化方法AC內毒素Not analyzed表達系統E.coli活性Not tested標簽C-His緩 沖 液20 mM Tris-HCl with 20% Glycerol保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產品介紹This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193產品圖片
Recombinant human IL-22 protein
基本售價：4800.00元
Recombinant human IL-22 protein產品編號bs-43572P英文名稱Recombinant human IL-22 protein中文名稱重組人白介素22蛋白性 狀Lyophilized or Liquid濃 度1mg/ml保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產品介紹產品圖片
Recombinant human IL-10 protein (Active, HEK293)
基本售價：2080.00元
Recombinant human IL-10 protein (Active, HEK293) 產品編號ck0006英文名稱Recombinant human IL-10 protein (Active, HEK293)中文名稱重組人白介素10 (活性的, Tag free)別 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重組蛋白; 人白細胞介素10 ; 重組人白介素10; 重組人IL-10;理論分子量17kDa檢測分子量21 kDa性 狀Lyophilized powder濃 度＞0.5 mg/ml物 種Human序 列19-178/178純 度>95% as determined by SDS-PAGE純化方法AC內毒素<0.5 EU/μg as determined by LAL.表達系統HEK293 cell活性Yes標簽Tag free緩 沖 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產品介紹Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586產品圖片
Recombinant human IL-15 protein (Active, HEK293)
基本售價：2080.00元
Recombinant human IL-15 protein (Active, HEK293) 產品編號ck0013產品分類重組蛋白英文名稱Recombinant human IL-15 protein (Active, HEK293)中文名稱重組人白介素15 (活性的, Tag free)英文別名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文別名人白介素15重組蛋白; 重組人白介素15; 人重組白介素15;純化方法AC檢測分子量14-15 kDaGeneID3600SwissP40933濃度＞0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白質與多肽-多肽序列49-162/162小分子偶聯蛋白-蛋白載體標簽Tag free純度>95% as determined by SDS-PAGE內毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達系統HEK293 cell復溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Recombinant human IL-2 protein (Active, HEK293)
基本售價：1080.00元
產品編號ck0017產品分類重組蛋白英文名稱Recombinant human IL-2 protein (Active, HEK293)中文名稱重組人白介素2 (活性的, Tag free)英文別名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文別名人白介素2重組蛋白; 重組人白介素2; 人重組白介素2; 重組人白介素2（IL-2）;蛋白細胞定位分泌型蛋白純化方法AC理論分子量15kDa檢測分子量17 kDaGeneID3558SwissP60568濃度＞0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景資料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白質與多肽-多肽序列21-153/153小分子偶聯蛋白-蛋白載體標簽Tag free純度>95% as determined by SDS-PAGE內毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達系統HEK293 cell復溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
人白介素15(IL-15)酶聯免疫吸附測定試劑盒
基本售價：3200.00元
Human IL-15(Interleukin 15) ELISA Kit文獻(2) 產品貨號:E-EL-H0222c 中文名:人白介素15(IL-15)酶聯免疫吸附測定試劑盒別稱:IL15用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中IL-15濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-15抗體包被于酶標板上，實驗時樣品或標準品中的人IL-15會與包被抗體結合，游離的成分被洗去。依次加入生物素化的抗人IL-15抗體和辣根過氧化物酶標記的親和素。抗人IL-15抗體與結合在包被抗體上的人IL-15結合、生物素與親和素特異性結合而形成免疫復合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現藍色，加終止液后變成黃色。用酶標儀在450nm波長處測OD值，IL-15濃度與OD450值之間呈正比，通過繪制標準曲線計算出樣品中IL-15的濃度。反應類型 夾心法規格 96T反應時間 3.5h反應性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人IL-15，且與其它相關蛋白無明顯交叉反應。重復性 板內，板間變異系數均<10%。典型數據 由于實驗操作條件的不同（如操作者、移液技術、洗板技術和穩定條件等），標準曲線的OD值會有所差異。以下數據和曲線僅供參考，實驗者需根據自己的實驗建立標準曲線。(pg/mL) O.D Average Corrected 10002.4852.5412.5132.4365001.6481.6681.6581.5812500.9160.8840.90.8231250.480.50.490.41362.50.2910.2710.2810.20431.250.1790.1770.1780.10115.630.1240.1340.1290.05200.0700.0840.077--精密度板內精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.35146.58348.0446.47145.32338.28Standarddeviation 3.028.2715.043.166.4718.37C V (%) 6.385.644.326.804.455.43回收率 分別往5個不同樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 92-10397Cell culture media (n=8) 88-10394線性 分別往5個樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)96-11096-10984-97Average (%)101102901:4Range (%)88-10183-9584-96Average (%)9588911:8Range (%)91-10581-9289-99Average (%)9987941:16Range (%)93-10488-10088-100Average (%)999394試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規格 保存條件 ELISA酶標板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標準品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標準品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產品說明書Product Description1 份質檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發和微生物的污染。試劑體積以實際發貨版說明書為準。相關試劑在分裝時會比標簽上標明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應板孔中加入100μL標準品工作液或樣本，37℃孵育90分鐘2. 棄掉板內液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內液體，洗板3次4. 每孔加入100μL HRP酶結合物工作液，37℃孵育30分鐘，棄掉板內液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數，處理數據
人白介素23(IL-23)酶聯免疫吸附測定試劑盒
基本售價：3200.00元
Human IL-23(Interleukin 23) ELISA Kit文獻(10) 產品貨號:E-EL-H0107c 中文名:人白介素23(IL-23)酶聯免疫吸附測定試劑盒別稱:IL-23A, IL23P19, P19, SGRF用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中IL-23濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-23抗體包被于酶標板上，實驗時樣品或標準品中的人IL-23會與包被抗體結合，游離的成分被洗去。依次加入生物素化的抗人IL-23抗體和辣根過氧化物酶標記的親和素。抗人IL-23抗體與結合在包被抗體上的人IL-23結合、生物素與親和素特異性結合而形成免疫復合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現藍色，加終止液后變成黃色。用酶標儀在450nm波長處測OD值，IL-23濃度與OD450值之間呈正比，通過繪制標準曲線計算出樣品中IL-23的濃度。反應類型 夾心法規格 96T反應時間 3.5h反應性 Human檢測方法 Colormetric檢測范圍 39.06—2500 pg/mL靈敏度 23.44 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人IL-23，且與其它相關蛋白無明顯交叉反應。重復性 板內，板間變異系數均<10%。典型數據 由于實驗操作條件的不同（如操作者、移液技術、洗板技術和穩定條件等），標準曲線的OD值會有所差異。以下數據和曲線僅供參考，實驗者需根據自己的實驗建立標準曲線。(pg/mL) O.D Average Corrected 25002.1472.1892.1682.11512501.4851.5191.5021.4496250.830.7920.8110.758312.50.3640.3740.3690.316156.250.230.2020.2160.16378.130.1480.1320.140.08539.060.0930.1050.0990.04600.0450.0610.053--精密度板內精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 136.57343.261072.26129.52356.171144.30Standarddeviation 7.2015.5535.498.4820.0254.47C V (%) 5.274.533.316.555.624.76回收率 分別往5個不同樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 88-10094EDTA plasma (n=8) 94-10599Cell culture media (n=8) 87-10093線性 分別往5個樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)92-10795-10985-99Average (%)99102911:4Range (%)93-10484-9686-102Average (%)9890931:8Range (%)91-10683-9487-99Average (%)9989941:16Range (%)88-10384-9480-92Average (%)958987試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規格 保存條件 ELISA酶標板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標準品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標準品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產品說明書Product Description1 份質檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發和微生物的污染。試劑體積以實際發貨版說明書為準。相關試劑在分裝時會比標簽上標明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應板孔中加入100μL標準品工作液或樣本，37℃孵育90分鐘2. 棄掉板內液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內液體，洗板3次4. 每孔加入100μL HRP酶結合物工作液，37℃孵育30分鐘，棄掉板內液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數，處理數據
人可溶性白介素6受體(sIL-6R)酶聯免疫吸附測定試劑盒
基本售價：3200.00元
Human sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit產品貨號:E-EL-H2518c 中文名:人可溶性白介素6受體(sIL-6R)酶聯免疫吸附測定試劑盒別稱:IL6R, CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin-6 receptor, interleukin 6 receptor用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中sIL-6R濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人sIL-6R抗體包被于酶標板上，實驗時樣品或標準品中的人sIL-6R會與包被抗體結合，游離的成分被洗去。依次加入生物素化的抗人sIL-6R抗體和辣根過氧化物酶標記的親和素。抗人sIL-6R抗體與結合在包被抗體上的人sIL-6R結合、生物素與親和素特異性結合而形成免疫復合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現藍色，加終止液后變成黃色。用酶標儀在450nm波長處測OD值，sIL-6R濃度與OD450值之間呈正比，通過繪制標準曲線計算出樣品中sIL-6R的濃度。反應類型 夾心法規格 96T反應時間 4.5h反應性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人sIL-6R，且與其它相關蛋白無明顯交叉反應。重復性 板內，板間變異系數均<10%。典型數據 由于實驗操作條件的不同（如操作者、移液技術、洗板技術和穩定條件等），標準曲線的OD值會有所差異。以下數據和曲線僅供參考，實驗者需根據自己的實驗建立標準曲線。(pg/mL) O.D Average Corrected 10002.4172.4692.4432.3845001.6951.7411.7181.6592500.9740.9390.9570.8981250.4350.4550.4450.38662.50.2680.2460.2570.19831.250.1750.1490.1620.10315.630.1060.1180.1120.05300.0490.0690.059--精密度板內精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 54.13124.28477.0256.05115.03491.14Standarddeviation 3.265.4422.943.755.2221.71C V (%) 6.024.384.816.694.544.42回收率 分別往5個不同樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 83-9590EDTA plasma (n=8) 87-9792Cell culture media (n=8) 83-9590線性 分別往5個樣本中添加已知濃度的目標蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)87-9998-11196-110Average (%)931051031:4Range (%)86-100101-11698-111Average (%)921071041:8Range (%)84-9797-10894-111Average (%)891021021:16Range (%)83-92100-11792-107Average (%)88108100試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規格 保存條件 ELISA酶標板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標準品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標準品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產品說明書Product Description1 份質檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發和微生物的污染。試劑體積以實際發貨版說明書為準。相關試劑在分裝時會比標簽上標明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應板孔中加入100μL標準品工作液或樣本，37℃孵育90分鐘2. 棄掉板內液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內液體，洗板3次4. 每孔加入100μL HRP酶結合物工作液，37℃孵育30分鐘，棄掉板內液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數，處理數據
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